A spectrophotometer is an analytical instrument used for the objective calculation of visible light, UV light, or infrared light emission or reflection. Spectrophotometers measure intensity as a function of the wavelength of the light source.

Spectrophotometry is a procedure for determining how much light is reflected by a chemical material by measuring the strength of light as a light beam travels through the sample solution. The fundamental theory is that light is absorbed or emitted over a certain wavelength spectrum by each compound.

A spectrometer is an aspect of the most responsible spectrophotometer for the calculation of different objects. A spectrophotometer is a comprehensive device that involves a light source, a way of collecting the light that has interacted with the objects being measured, and a measurement spectrometer.

The source of light is given by a lamp. The light beam strikes the diffraction grating, which acts like a mirror and divides the light into the wavelengths of its elements. The grating is rotated such that the exit slit is only penetrated by a single wavelength of light. Then with the sample, the light interacts.

A blank is a sample containing everything except for the significance analyte. For example, if you are conducting an experiment with UV-Vis to measure Green Fluorescent Protein concentrations, the protein must be dissolved in a solvent. The blank is a sample of the solvent itself.